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Glaucoma Research
We
heard of the work of a Dr Lohi in Finland via Minna Repo and
Margreth Wall, the Finnish Dandie club, small as they are have
been holding events to raise funds and have done well
considering their size. We looked into this work and found
that Dr Lohi is also working in conjunction with the Uni of
Missouri who we have funded in the past, trouble is with that
research we had to piggy back on the backs of other bigger
breeds because they could raise more money - for obvious
reasons.
Dr
Lohi seems to have a particular interest in dandies and so far
we have sent $6000 with more to come, and obviously, we have
to keep his interest alive and continue to fund his research
to help our dandies, it cannot be a 'one off' thing or we lose
it, anyway I send you part of a mail he sent us which will
give you a better understanding -
January 2009
Dear Celia,
Saija has analyzed the genotyping data and it looks very
promising. We
have a single locus on one chromosome and it is significant so
we
believe it! This means we have mapped the Dandie's
glaucoma gene but
have to work now hard to identify the actual mutation. This
requires
lots of resequencing and it would help us a lot if you could
support
us again financially and especially by collecting as many
dandies as
possible for our DNA bank. Resequencing is likely 10-15
000 euros.
Very exciting!
Thanks.
Hannes
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Dear
Celia,
We have collected Dandies here Finland and in US (Dr. Gary
Johnson) and
continue doing so. Any samples from UK or other countries
would be
helpful. We have currently 20 affected and 90 unaffected
samples and we
would like to perform the first round of genotyping to map the
glaucoma
locus in this breed. We have several litters with multiple
affecteds
increasing the power of our study. I would specifically ask
funds for
this mapping project. We would use the newest high-resolution
marker
technology with Illumina's SNP chip arrays. The cost for one
chip is
272$US. For 40 samples it would make 10 880$ + shipping.
Together with
Gary, we would run experiments here in Finland in the Finnish
Genome
Center, where I have a special access. Since we already have
the DNAs
for 40 samples, we could start immediately. It would be
awesome if you
could support us to buy those chips. Finnish DandiClub has
collected
~1000? so far for this project, so if you could supplement the
rest, we
could start mapping the locus. If 10 000$ is not in the scope
of your
funding opportunities, I would suggest that we gebotype 20
cases and 10
controls initially. This reduces the power only modestly but
we could
perform this for 8160$. Actually if I knew your commitment for
donation
before next Wednesday we could save even more. May 30th we
have a
special offer from Illumina for the European Canine Consortium
I belong,
it is 218$/sample: 40= 8720$ and 6540$.
I
believe that we should proceed as described before to map and
develop
marker for your condition in Dandies. Meanwhile we try to
expand sample
collection around world. I believe we have a great chance to
be
successful even in this first round of mapping but if not we
will simply
genotype more dogs collected during this time.
Another
project I would like to do for your breed relates to
DLA-typing.
We can DLA type the same samples we use for glaucoma . DLA
typing tells
about the genetic diversity within your breed and certain DLA
classes
can be associated with different autoimmune diseases. I can
perform
these at the same time as glaucoma mapping proceeds.
Hannes Lohi, Docent
Academy Research Fellow
Biomedicum Helsinki, Room B336b
Department of Medical Genetics,Program in Molecular Medicine
University of Helsinki and the Folkhälsan Institute of
Genetics
P.O.Box 63 (Haartmaninkatu 8)
00014 University of Helsinki
phone +358-9-191 25085
fax +358-9-191 25073
www.koirangeenit.fi
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